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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Medawar A, et al. 2008. PLoS ONE 3:e3441. PubMed 2. Di Girolamo N, et al. 2009. Transplantation. 87:1571. PubMed
Immunofluorescent microscope analysis of Hela cells using anti-p63 ΔN polyclonal antibody (poly6190) (red). a-tubulin (clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue).
Non-transfected 293 cell lysates (Lane 1) and p63 (deltaN) transfected 293 cell lysates (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p63 (deltaN) antibody (clone Poly6190). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
p63 (ΔN) is a member of the p53 family. This protein has multiple isoforms TA p63-α, -β, γ, ΔN p63-α, -β, and -γ. The molecular weights of the p63 isoforms α=66 kD, β=52 kD, and γ=45 kD. This nuclear protein is essential for limb formation, epidermal morphogenesis, and epithelial stem cell regeneration. ΔN isoforms are antagonistic to p53; TA isoforms can transactivate p53 targets. The p63 ΔN isoforms repress TA isoforms; mutant p53 abrogates p63 transactivation. This protein interacts with p53, p73, and the various TA- and ΔN isoforms. The Poly6190 antibody recognizes human p63 ΔN isoforms and has been shown to be useful for Western blotting.
Other Names:
p63, ΔN p63-α, -β, -γ
Structure:
p53 family, six isoforms, ΔN p63α, β, γ, approximately 66 kD, 52 kD, 45 kD
Distribution:
Nuclear
Function:
Essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration. ΔN isoforms antagonistic to p53; TA isoforms can transactivate p53 targets
Regulation:
ΔN isoforms repress TA isoforms, mutant p53 abrogates p63 transactivation
Interaction:
p53, p73, TA- and ΔN isoforms
Antigen References:
1. Yang A, et al. 1998. Mol Cell. 2:305. 2. Celli J, et al. 1999. Cell. 99:143. 3. Gaiddon C, et al. 2001. Mol Cell Biol. 21:1874. 4. Benard J, et al. 2003. Hum Mutat. 21:182.
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Purified anti-p63 (ΔN)
Immunofluorescent microscope analysis of Hela cells using anti-p63 ΔN polyclonal antibody (poly6190) (red). a-tubulin (clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue).
Non-transfected 293 cell lysates (Lane 1) and p63 (deltaN) transfected 293 cell lysates (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p63 (deltaN) antibody (clone Poly6190). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
