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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The optimal concentration should be determined by titration for each individual assay of interest.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with polyclonal anti-p62 antibody (Clone Poly6477). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
The p62 protein, also called sequestosome 1 (SQSTM1), is a ubiquitin-binding scaffold protein that colocalizes with ubiquitinated protein aggregates in many neurodegenerative diseases and proteinopathies of the liver. The protein is able to polymerize via an N-terminal PB1 domain and can interact with ubiquitinated proteins via the C-terminal UBA domain. Also, p62/SQSTM1 binds directly to LC3 and GABARAP family proteins via a specific sequence motif. The protein is itself degraded by autophagy and may serve to link ubiquitinated proteins to the autophagic machinery to enable their degradation in the lysosome. Recent studies have revealed a novel function for p62 in innate immunity.
Other Names:
Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kD, Ubiquitin-binding protein p62
Structure:
An N-terminal PB1 domain, a C-terminal UBA domain,62kD
Distribution:
Cytosolic protein inclusions, nuclear and cytosolic compartments, nuclear PML (promyelocytic leukemia) bodies
Interaction:
polymerization, ubiquitinated proteins, LC3 and GABARAP family proteins
Antigen References:
1. Falchetti A, et al. 2005. Arthritis Res Ther. 7:R1289. 2. Bjørkøy. G, et al. 2005. J.Cell Biol. 171:603. 3. Pankiv S, et al. 2007. J. Biol Chem. 282:24131. 4. Zheng YT, et al. 2009. J. Immunol. 183:5909.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-P62
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with polyclonal anti-p62 antibody (Clone Poly6477). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
