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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Yap DBS, et al. 2004. Cancer Res. 64:4749. (WB IHC)
Panel A shows western blot analysis of extracts from untreated MCF-7 cells (Lane 1) or VP-16 treated MCF-7 cells (68 mM VP-16 for 6 hrs) (Lane 2), using anti-phospho-p53 (Ser392), clone FP3.2. Panel B shows the same blot re-probed with a pan-p53 specific monoclonal antibody (BP53-12).
p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73. The FP3.2 monoclonal antibody recognizes human phosphorylated p53 (Ser392) and has been shown to be useful for Western blotting and immunohistochemistry (paraffin).
Other Names:
Tumor protein p53 (TP53)
Structure:
Tetramer; 53 kD
Distribution:
Nuclear when activated
Function:
Tumor suppressor, transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, cell adhesion
Regulation:
Ubiquinated for degradation via mdm2. Upregulated by genotoxic or other cellular stresses
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-p53-Phosphorylated (Ser392)
Panel A shows western blot analysis of extracts from untreated MCF-7 cells (Lane 1) or VP-16 treated MCF-7 cells (68 mM VP-16 for 6 hrs) (Lane 2), using anti-phospho-p53 (Ser392), clone FP3.2. Panel B shows the same blot re-probed with a pan-p53 specific monoclonal antibody (BP53-12).
