This antibody is provided in Tris-buffered solution, pH 8.0, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation:
This antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C.
Application:
WB - Quality Tested IP*, ICC*, ELISA * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-p53 (clone BP53-12) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Description:
p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.The BP53-12 monoclonal antibody recognizes human and non-human primate p53 and has been shown to be useful for Western blotting, immunoprecipitation, immunohistochemistry, immunocytochemistry, and ELISA.
Other Names:
Tumor protein p53 (TP53)
Structure:
Tetramer; 53 kD
Distribution:
Nuclear when activated
Function:
Tumor suppressor, transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, cell adhesion
Regulation:
Ubiquinated for degradation via mdm2. Upregulated by genotoxic or other cellular stresses
