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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Wang, et al. 2003. J. Biol. Chem. 278:25568
MCF-7 cells were treated with 50 µM sodium butyrate and 2 nM actinomycin D for 8 hrs. MCF7 cell extract was resolved by electrophoresis (panel B) or immunoprecipitated with a p53 monoclonal (panel A) and resolved by electrophoresis. Proteins were transferred to nitrocellulose, and probed with rabbit polyclonal antibody against acetylated p53 (Lys382). Lane 1=untreated, control MCF-7 cells; Lane 2=treated MCF-7 cells. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73. The Poly6142 antibody recognizes human acetylated p53 (Lys382) and has been shown to be useful for Western blotting.
Other Names:
Tumor protein p53 (TP53)
Structure:
Tetramer; 53 kD
Distribution:
Nuclear when activated
Function:
Tumor suppressor, transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, cell adhesion
Regulation:
Ubiquinated for degradation via mdm2. Upregulated by genotoxic or other cellular stresses
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Purified anti-p53-Acetylated (Lys382)
MCF-7 cells were treated with 50 µM sodium butyrate and 2 nM actinomycin D for 8 hrs. MCF7 cell extract was resolved by electrophoresis (panel B) or immunoprecipitated with a p53 monoclonal (panel A) and resolved by electrophoresis. Proteins were transferred to nitrocellulose, and probed with rabbit polyclonal antibody against acetylated p53 (Lys382). Lane 1=untreated, control MCF-7 cells; Lane 2=treated MCF-7 cells. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
