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Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Mandriota SJ, et al. 2010. J. Biol Chem. 285:13092. PubMed
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-p53 (clone BP53-12) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.The BP53-12 monoclonal antibody recognizes human and non-human primate p53 and has been shown to be useful for Western blotting, immunoprecipitation, immunohistochemistry, immunocytochemistry, and ELISA.
Other Names:
Tumor protein p53 (TP53)
Structure:
Tetramer; 53 kD
Distribution:
Nuclear when activated
Function:
Tumor suppressor, transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, cell adhesion
Regulation:
Ubiquinated for degradation via mdm2. Upregulated by genotoxic or other cellular stresses
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-p53
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-p53 (clone BP53-12) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
