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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-p38 MAPK antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Formalin-fixed paraffin-embedded human placenta tissue was stained with Poly6224 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
p38 mitogen-activated protein kinase (MAPK) is a member of the stress activated kinase family with a molecular weight of 38 kD. MAPK can be activated by a number of stimuli including heat, changes in osmolarity, irradiation, and cold. The MAPKs require dual phosphorylation (threonine and tyrosine) for enzymatic activation. The p38 MAP kinase is required for G2 cell cycle delay after UV irradiation, also participates in cytokine production (IL-6 and erythropoietin). The Poly6224 antibody recognizes human, mouse, and rat p38 MAPK and has been shown to be useful for Western blotting.
Belongs to serine/threonine kinase family, MAP subfamily, 38 kD
Distribution:
Ubiquitously expressed, nuclear and cytoplasmic
Function:
p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knock-out studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2 an
Regulation:
Activated by environmental stress, many pro-inflammatory cytokines and lipopolysaccharide. Dual phosphorylation by MAP2K3 and MAP2K6 is required for activation of p38 MAPK.
Modification:
Phosphorylation
Interaction:
Interacts with MAX, Cdc25B, Cdc25C. Binds to kinase interaction domain in the protein tyrosine phosphatase PTPRR; this interaction retains p38 MAPK in the cytoplasm.
Antigen References:
1. Bulavin DV, et al. 2001. Nature 411:102. 2. Han J, et al. 1994. Science 265:808. 3. Lee JC, et al. 1994. Nature 372:739.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-p38 MAPK
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-p38 MAPK antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Formalin-fixed paraffin-embedded human placenta tissue was stained with Poly6224 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
