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Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy: Use a starting dilution of 1:100. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Immunofluorescent microscope analysis of NTERA-2 cells using anti-Oct4 polyclonal antibody (poly6319) (red). α-tubulin (clone 10D8) has been labeled with green, and nuclei were stained with DAPI (blue).
Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and a central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct-4 is downregulated thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is nucleus protein, with a predicted molecular weight 39 kD. The poly6318 antibody recognizes human Oct4 and has been shown to be useful for immunofluorescent staining.
Other Names:
POU domain class 5, transcription factor 1, POU5F1, Oct3, Oct-3
Structure:
Belongs to the POU transcription factor family, predicted molecular weight 39 kD
Distribution:
Nucleus protein, express in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells
Function:
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and a central regulator of pluripotency in ES cells.
Interaction:
SOX2, SOX8, SOX10, androgen receptor and ETS2
Antigen References:
1. Niwa H, et al. 2000. Nature Genet. 24:372. 2. Wernig M, et al. 2007. Nature 448:318. 3. Maurizio P,et al. 2001. Stem Cells 19:271.
Purified anti-Oct4 (Oct-4), Poly6319, Purified, POU domain class 5, transcription factor 1, POU5F1, Oct3, Oct-3 , Human, Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Oct4 (Oct-4)
Immunofluorescent microscope analysis of NTERA-2 cells using anti-Oct4 polyclonal antibody (poly6319) (red). α-tubulin (clone 10D8) has been labeled with green, and nuclei were stained with DAPI (blue).
