Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy: Use a starting dilution of 1:100. It is recommended that the reagent be titrated for optimal performance for each application.
Immunofluorescent microscope analysis of NTERA-2 cells using anti-Oct4 polyclonal antibody (poly6319) (red). α-tubulin (clone 10D8) has been labeled with green, and nuclei were stained with DAPI (blue).
Description:
Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and a central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct-4 is downregulated thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is nucleus protein, with a predicted molecular weight 39 kDa. The poly6318 antibody recognizes human Oct4 and has been shown to be useful for immunofluorescent staining.
Other Names:
POU domain class 5, transcription factor 1, POU5F1, Oct3, Oct-3
Structure:
Belongs to the POU transcription factor family, predicted molecular weight 39 kDa
Distribution:
Nucleus protein, express in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells
Function:
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and a central regulator of pluripotency in ES cells.
Interaction:
SOX2, SOX8, SOX10, androgen receptor and ETS2
Antigen References:
1. Niwa, H., et al. 2000. Nature Genet. 24:372. 2. Wernig, M., et al. 2007. Nature 448:318. 3. Maurizio P.,et al. 2001. Stem Cells 19:271.
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