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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 15 µl/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Brdicka T, et al. 2002. J. Exp. Med. 196:1617.
Formalin-fixed paraffin-embedded human tonsil tissue was stained with NAP-07 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Extracts from peripheral blood mononuclear cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse monoclonal antibody against NTAL. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
NTAL is a transmembrane adaptor protein with an apparent molecular weight of 30 kD expressed on B cells, NK cells, and myeloid cells. The NTAL is a transmembrane adaptor protein found in lipid raft microdomains that participates in B cell activation. NTAL interacts with a variety of proteins including Grb2, PLC gamma 2, and PLC gamma 1. The NAP-07 monoclonal antibody reacts with human and mouse NTAL and has been shown to be useful for Western blotting.
Other Names:
Non-T cell activation linker, linker of activated B cells (LAB), Williams-Beuren syndrome chromosome region 5
Structure:
Transmembrane adaptor protein, 30 kD
Distribution:
B cells, NK cells, myeloid cells
Function:
Adaptor protein found in lipid raft microdomains, participates in B cell activation
Modification:
Potential phosphorylation and palmitoylation
Interaction:
Grb2, PLC gamma 2, PLC gamma 1
Antigen References:
1. Doyle JL, et al. 2000. Cytogenet. Cell Genet. 90:285. 2. Janssen E, et al. 2003. Nature Immun. 4:117.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-NTAL
Formalin-fixed paraffin-embedded human tonsil tissue was stained with NAP-07 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Extracts from peripheral blood mononuclear cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse monoclonal antibody against NTAL. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
