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Human, reacts against Thr234/Thr237-phosphorylated NPM/B23
Immunogen:
Modified peptide
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. Bovine serum albumin is present at 1 mg/ml final concentration (USA origin).
Preparation:
The antibody was purified by antigen-affinity chromatography.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Hela cells were treated with 300 µM mimosine for 16 h, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18h. Nuclear extract was western blotted with Poly6191. In addition to the specific NPM/B23 band, this antibody recognizes several other non-specific proteins with molecular weights >66kD.
Formalin-fixed paraffin-embedded human epidermis tissue was stained with Poly6191 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
NPM/B23 (also known as numatrin, nucleophosmin 1, nucleolar phosphoprotein B23) is a member of the nucleophosmin/nucleoplasmin family that shuttles between the nucleus and cytoplasm. This protein regulates the stability and transcriptional activity of p53 and acts as a molecular chaperone. NPM/B23 preferentially binds to denatured proteins and has been shown to stimulate DNA polymerase activity and control the duplication of centrosomes. The chaperone activity of NPM/B23 is regulated by protein kinase CK2 and promotes release of denatured substrates from NPM/B23. NPM/B23 is modified by phosphorylation onThr199. This protein has been shown to bind to RNA and DNA and interact with nucleolar proteins including nucleolin, protein P120, HIV-1 Rev protein, and hepatitis delta antigens. The Poly6191 antibody recognizes human phosphorylated NPM/B23 (Thr234/Thr237) and has been shown to be useful for Western blotting.
Nucleolar protein, can shuttle between nucleus and cytoplasm
Function:
Regulates the stability and transcriptional activity of p53, acts as a molecular chaperone. Preferentially binds to denatured proteins. Has been shown to stimulate DNA polymerase α activity and control the duplication of centrosomes
Regulation:
Chaperone activity is regulated by protein kinase CK2, promotes release of denatured substrates from NPM/B23
Modification:
Phosphorylation
Interaction:
Binds to RNA, DNA, and interacts with nucleolar proteins including nucleolin, protein P120, HIV-1 Rev protein, and hepatitis delta antigens
Antigen References:
1. Szebeni A, et al. 2003. J. Biol. Chem. 278:9107. 2. Colombo E, et al. 2002. Nat. Cell Biol. 4:529. 3. Chan PK, et al. 1986. J. Biol. Chem. 261:14335.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Hela cells were treated with 300 µM mimosine for 16 h, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18h. Nuclear extract was western blotted with Poly6191. In addition to the specific NPM/B23 band, this antibody recognizes several other non-specific proteins with molecular weights >66kD.
Formalin-fixed paraffin-embedded human epidermis tissue was stained with Poly6191 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
