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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Cell extract from MCF-7 cells transfected with either human Nod1 (Lane 1) or human Nod2 (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-Nod2 antibody (clone NOD-15). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. The NOD-15 antibody is specific for human Nod2 and does not cross-react with human Nod1.
Nod2, also known as caspase recruitment domain-containing protein 15, CARD15, and inflammatory bowel disease protein 1, is a monocyte-specific cytoplasmic protein containing two N-terminal caspase recruitment domains (CARD domains), and multiple leucine-rich repeats with a predicted molecular weight 115 kD. Alternative initiation produces two isoforms of Nod2. Nod2 is thought to confer responsiveness to intracellular bacterial lipopolysaccharide (LPS), and defects in Nod2 have been linked to several diseases including Blau syndrome, Crohns's disease, and ulcerative colitis. Knock-out animals has been shown to have dramatically altered responses to bacterial pathogens in the gut. Nod2 binds to the receptor interacting kinase 1 protein via CARD-CARD interaction and induces NF-κB and IKK-gamma. The NOD-15 monoclonal antibody recognizes the human Nod2 protein and has been shown to be useful for Western blotting. The NOD-15 antibody does not cross-react with human Nod1.
Other Names:
Caspase recruitment domain-containing protein 15, CARD15, inflammatory bowel disease protein 1
Structure:
Cytoplasmic protein containing two N-terminal caspase recruitment domains (CARD domains), multiple leucine-rich repeats, predicted molecular weight 115 kD. Alternative initiation produces two isoforms.
Distribution:
Monocyte-specific
Function:
Confers responsiveness to intracellular bacterial lipopolysaccharide (LPS). Binds to RIP2 via CARD-CARD interaction and induces NF-κB and IKK-gamma. Defects in Nod1 are associated with Blau syndrome, Crohn's disease, and ulcerative colitis.
Interaction:
RIP2, CARD12
Antigen References:
1. Ogura Y, et al. 2001. J. Biol. Chem. 276:4812. 2. Hugot JP, et al. 2001. Nature 411:599. 3. Miceli-Richard C, et al. 2001. Nat. Genet. 29:19. 4. Kobayashi KS, et al. 2005. Science 37:731.
Purified anti-Nod2, NOD-15, Purified, Caspase recruitment domain-containing protein 15, CARD15, inflammatory bowel disease protein 1, Human Nod2, does not cross-react with Nod1, Immunology, Antibodies
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Purified anti-Nod2
Cell extract from MCF-7 cells transfected with either human Nod1 (Lane 1) or human Nod2 (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-Nod2 antibody (clone NOD-15). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. The NOD-15 antibody is specific for human Nod2 and does not cross-react with human Nod1.
