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Purified Anti-Ninein Antibody
Material Safety Data Sheet (MSDS) Purified Anti-Ninein Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-Ninein Antibody
602801 50 µl (5 Western blots) $75.00     
602802 200 µl (20 Western blots) $225.00     
Clone: Poly6028
Isotype: Rabbit IgG
Reactivity: Human
Immunogen: Recombinant (partial), N-terminal
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20° C.
Application:

WB

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application
References:

1. Chang, B., et al., 2006. Human Molecular Genetics 15(11):1847.PubMed
2. Fuentabela, L.,et al.2007. Cell.131:980.PubMed
3. Matsumoto, T.,et al. 2008. Arterisocler Thromb Vasc Biol.10:1161..PubMed

MCF7 (Lane 1) and MOLT-4

MCF7 (Lane 1) and MOLT-4 (Lane 2) cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-ninein antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In hematopoietic cells (such as MOLT-4), two additional bands with apparent molecular weights of 110 and 130 kD are observed.


Description:

Ninein (also known as GSK3β-interacting protein and ninein centrosomal protein) is a chromosome segregation ATPase with 5 reported isoforms, (molecular weights ranging from 236 to 246 kD). Ninein is localized at the centrosome and is thought to position and anchor microtubules minus-ends. Ninein is important for centrosomal function in epithelial cells. This protein is modified by phosphorylation. Ninein has also been shown to interact with CEP250/c-Nap1, CEP110, γ-tubulin, centrin, GSK3β, AIK, and PKA. The Poly6028 antibody recognizes the N-terminal region of human ninein and has been shown to be useful for Western blotting.

Other Names: GSK3β-interacting protein, Ninein centrosomal protein
Structure: Chromosome segregation ATPases; 5 isoforms, 236/246/241/244/243 kD
Distribution: Centrosomal
Function: Positions and anchors microtubules minus-ends. Important for centrosomal function in epithelial cells
Modification: Phosphorylation
Interaction: CEP250/c-Nap1, CEP110, γ-tubulin, centrin, GSK3β, AIK, PKA
Antigen
References:

1. Bouckson-Castaing, V., et al., 1996. J. Cell Sci. 109:179.
2. Hong, Y., et al., 2000. Biochim. Biophys. Acta 1492:513.
3. Ou, Y., et al., 2002. J. Cell. Sci. 115:1825.
4. Chen, C., et al., 2003. Biochem. Biophys. Res. Commun. 308:975.

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HRP Donkey anti-rabbit IgG (minimal x-reactivity)Poly4064ELISA,IHC,WB
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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