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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Chang B, et al. 2006. Human Molecular Genetics 15(11):1847.PubMed 2. Fuentabela L,et al.2007. Cell.131:980.PubMed 3. Matsumoto T,et al. 2008. Arterisocler Thromb Vasc Biol.10:1161.PubMed 4. Hurd T, et al. 2010. Hum Mol Genet. 19:4330. PubMed 5. Ibi M, et al. 2011. J Cell Sci. 124:857. PubMed
MCF7 (Lane 1) and MOLT-4 (Lane 2) cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-ninein antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In hematopoietic cells (such as MOLT-4), two additional bands with apparent molecular weights of 110 and 130 kD are observed.
Ninein (also known asGSK3β-interacting protein and ninein centrosomal protein) is a chromosome segregation ATPase with 5 reported isoforms, (molecular weights ranging from 236 to 246 kD). Ninein is localized at the centrosome and is thought to position and anchor microtubules minus-ends. Ninein is important for centrosomal function in epithelial cells. This protein is modified by phosphorylation. Ninein has also been shown to interact with CEP250/c-Nap1, CEP110, γ-tubulin, centrin, GSK3β, AIK, and PKA. The Poly6028 antibody recognizes the N-terminal region of human ninein and has been shown to be useful for Western blotting.
Other Names:
GSK3β-interacting protein, Ninein centrosomal protein
1. Bouckson-Castaing V, et al. 1996. J. Cell Sci. 109:179. 2. Hong Y, et al. 2000. Biochim. Biophys. Acta 1492:513. 3. Ou Y, et al. 2002. J. Cell. Sci. 115:1825. 4. Chen C, et al. 2003. Biochem. Biophys. Res. Commun. 308:975.
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Purified anti-Ninein
MCF7 (Lane 1) and MOLT-4 (Lane 2) cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-ninein antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In hematopoietic cells (such as MOLT-4), two additional bands with apparent molecular weights of 110 and 130 kD are observed.
