This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
MCF7 (Lane 1) and MOLT-4 (Lane 2) cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-ninein antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In hematopoietic cells (such as MOLT-4), two additional bands with apparent molecular weights of 110 and 130 kD are observed.
Description:
Ninein (also known asGSK3β-interacting protein and ninein centrosomal protein) is a chromosome segregation ATPase with 5 reported isoforms, (molecular weights ranging from 236 to 246 kD). Ninein is localized at the centrosome and is thought to position and anchor microtubules minus-ends. Ninein is important for centrosomal function in epithelial cells. This protein is modified by phosphorylation. Ninein has also been shown to interact with CEP250/c-Nap1, CEP110, γ-tubulin, centrin, GSK3β, AIK, and PKA. The Poly6028 antibody recognizes the N-terminal region of human ninein and has been shown to be useful for Western blotting.
Other Names:
GSK3β-interacting protein, Ninein centrosomal protein
