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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5 µl in 5 ml blocking buffer (0.2 µg/ml). It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Personal communication (WB, IF)
Protein extracts prepared from wild-type mouse bone marrow derived macrophages (BMMs) incubated without (-R, Lane 1, BMMs) or with RANKL (+R, Lane 2, Osteoclasts) were resolved by electrophoresis. The membrane was probed with polyclonal anti-Nhedc2 antibody. Protein was visualized by goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. This antibody recognizes Nhedc2, which runs at approximately 50 kD with predicted molecular weight of 58 kD.
The sodium-proton exchangers (NHE) antiporters of the CPA1 family, catalyze Na+ and H+ exchange. NHE activity is also involved in other cellular events such as cell migration, adhesion and proliferation in response to growth factors. Nhedc2 is a novel member of the CPA family and is the first mitochondrial NHA characterized to date. It is also unique in that it is the first eukaryotic and tissue-specific CPA2 characterized to date. Nhedc2 is localized to the mitochondria, where it mediates Na+-dependent exchanges in mitochondrial PH and Na+ acetate induced mitochondrial passive swelling. It is strongly upregulated during RANKL-induced osteoclast differentiation in vivo and in vitro. RNA silencing of this gene reduces osteoclast differentiation and resorption, suggesting that it plays a key role(s) in normal osteoclast differentiation and function.
Nhedc2 encodes a novel cation-proton antiporter (CPA). This protein localizes to the mitochondrion membrane.
Function:
Electroneutral exchange of protons for Na(+) and Li(+) across the inner mitochondrial membrane. It contributes to the organellar volume homeostasis. This protein may be important for osteoclast differentiation and bone resorption activity.
Antigen References:
1. Battaglino RA, et al. 2008. Bone 42:180. 2. Lee SH, et al. 2008. Biochem. Biophys. Res. Commun.369:320.
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Purified anti-Nhedc2 (NHA2)
Protein extracts prepared from wild-type mouse bone marrow derived macrophages (BMMs) incubated without (-R, Lane 1, BMMs) or with RANKL (+R, Lane 2, Osteoclasts) were resolved by electrophoresis. The membrane was probed with polyclonal anti-Nhedc2 antibody. Protein was visualized by goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. This antibody recognizes Nhedc2, which runs at approximately 50 kD with predicted molecular weight of 58 kD.
