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Each lot of this antibody is quality control tested by Western blotting. For Western blotting applications, a concentration of 1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
7A6 antibody detects endogenous human NFATC1 in Western blot with an approximate molecular weight of 100 - 120 kD (different isoforms). The optimal concentration should be determined by titration for each individual assay of interest.
Application References:
1. Timmerman LA, et al. 1997. J. Immunol. 159:2735. (IF) 2. Brandt C, et al. 2010. Cytometry A. 77:607. (FC)
Jurkat (Lane 1), human Th1 (Lane 2), and mouse Th1 (Lane 3) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-NFATc1 antibody (clone 7A6). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. The protein complex consists of NFAT1, NFAT2 (NFATc1 or NFATc), NFAT3 and NFAT4. All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response. NFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes and adipocytes, regulating cell differentiation programs in these cells. In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus.
Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
943 amino acids with predicted molecular weight of 101243 Da.
Distribution:
Cytoplasm, Nucleus. Note: NFATc1 is cytoplasmic in the phosphorylated form and nuclear after calcineurin-mediated dephosphorylation controlled activation.
Antigen References:
1. Zhao Q, et al. 2010. Int. J. Biochem. Cell B. 42:576. 2. Hoey T, et al. 1995. Immunity 2:461. 3. Northrop JP, et al. 1993. J. Biol. Chem. 268:2917. 4. Hogan PG, et al. 2003. Genes Dev. 17:2205. 5. Shaw KT, et al. 1995. P. Natl. Acad. Sci. USA 92:11205. 6. Serfling E, et al. 2007. Sci. STKE 138:42.
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Purified anti-NFATc1
Jurkat (Lane 1), human Th1 (Lane 2), and mouse Th1 (Lane 3) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-NFATc1 antibody (clone 7A6). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
