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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Leitch D, et al. 2003. Semin Oncol. 30:161. (IHC) 2. Stjernholm-Vladic Y, et al. 2004. Reprod. Biol. & Endo. 2:74. (IHC) 3. Popov Y, et al. 2006. J. Biol. Chem. 281:15090. (WB)
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-NF-κB p65 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Hela cells stained with purified rabbit polyclonal antibody against NF-κB p65 (poly6226), followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI
NF-κB/p65 (nuclear factor kappa light chain enhancer in B cells p65) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p65 is bound (with p50) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF-κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The Poly6226 antibody reacts with the C-terminal region of human, mouse, and rat NF-κB p50 and has been shown to be useful for Western blotting.
Other Names:
NFκB p65, nuclear factor kappa light chain enhancer in B cells p65
Structure:
Member of the Rel/dorsal family. One subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits derived from larger precursor proteins
Distribution:
Ubiquitously expressed nuclear protein
Function:
Transcriptional activator, originally identified as an activator of kappa light chain in B cells
Regulation:
Bound (with p50) to IkB inhibitor in cytoplasm in inactive form. Phosphorylation of IkB releases and NF-kB goes to the nucleus to activate gene expression. Stimulation with LPS, TNF-α, phorbol ester, or IL-1 activates
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Purified anti-NF-κB p65
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-NF-κB p65 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Hela cells stained with purified rabbit polyclonal antibody against NF-κB p65 (poly6226), followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI
