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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Tan BCM and Lee SC. 2004. J. Biol. Chem. 279:9321. (IHC, IP)
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-NEK9 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Formalin-fixed paraffin-embedded human brain cortex tissue was stained with Poly6241 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
NEK9 is a 107 kD member of the serine/threonine-protein kinase family, NIMA subfamily containing a coiled-coil domain, and RCC1 repeats. This cytoplasmic protein forms dimers and is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation, Nek9 is activated during mitosis by intramolecular autophosphorylation at Thr210. Nek9 has been shown to interact with Ran GTPase (greater affinity for Ran-GDP over Ran-GTP), NEK6, NEK7, BICD2, histone H3, myelin basic protein, and β-casein. The Poly6241 antibody recognizes human NEK9 and has been shown to be useful for Western blotting, immunohistochemistry, and immunoprecipitation.
Other Names:
Serine/Threonine-protein kinase Nek9, NimA-related protein kinase 9
Structure:
Serine/Threonine protein kinase family, NIMA subfamily, coiled-coil domain, RCC1 repeats, dimer; 107 kD
Distribution:
Cytoplasm
Function:
Pleiotropic regulator of mitotic progression, participates in control of spindle dynamics and chromosome separation
Regulation:
Activated during mitosis by intramolecular autophosphorylation
Interaction:
Ran GTPase, greater affinity for Ran-GDP over Ran-GTP, NEK6, NEK7, BICD2, histone H3, myelin basic protein, β-casein
Antigen References:
1. Holland P, et al. 2002. J. Biol. Chem. 277:16229. 2. Roig J, et al. 2002. Genes Dev. 16:1640. 3. Belham C, et al. 2003. J. Biol. Chem. 278:34897.
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Purified anti-NEK9
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-NEK9 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Formalin-fixed paraffin-embedded human brain cortex tissue was stained with Poly6241 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
