This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-NCOA3 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
NCOA3/SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. NCOA3/SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. NCOA3/SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. NCOA3/SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by IκB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. NCOA3/SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF. The Poly6092 antibody recognizes the N-terminal region of human NCOA3/SRC3 and has been shown to be useful for Western blotting
p160/steroid receptor coactivator family, nuclear localization sequence, bHLH, PAS domains; two isoforms 155 kD
Distribution:
Mainly cytoplasmic, weakly nuclear; translocated to nucleus upon phosphorylation. Widely expressed, high expression in the heart, skeletal muscle, pancreas, placenta; low expression in the brain, very low expression in the lung, liver, kidney; overexpress
Function:
Histone acetyltransferase activity, transcriptional coactivator for steroid and nuclear hormone receptors
Regulation:
Phosphorylation by IκB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors
Modification:
Phosphorylation, Acetylation
Interaction:
Forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein, interacts with PCAF
Antigen References:
1. Takeshita, A., et al., 1997. J. Biol. Chem. 272:27629. 2. Li, H., et al., 1997. PNAS 94:8479. 3. Wu, R., et al., 2002. Mol. Cell. Biol. 22:3549.
