Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-NCOA2/GRIP1 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
NCOA2/GRIP1 (nuclear receptor coactivator 2/glucocorticoid receptor-interacting protein 1 (GRIP1)) is a 159 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. Two isoforms of this protein have been reported. NCOA/GRIP1 is expressed in the cytoplasm and translocated into the nucleus upon phosphorylation. This protein is highly expressed in the placenta, skeletal muscle, uterus with lower expression in B cells, lymphocytes, and some tumors. NCOA/GRIP1 has histone acetyltransferase activity, and is a transcriptional coactivator for steroid and nuclear hormone receptor. This protein mediates ligand-independent nuclear translocation and activates the CAR receptor in response to phenobarbital. This protein is modified by phosphorylation. NCOA/GRIP1 forms a multisubunit coactivation complex with NCOA3, IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with HIF1A, APEX, NR3C1, and CAR. The Poly6090 antibody recognizes the N-terminal region of human NCOA2/GRIP1 and has been shown to be useful for Western blotting.
Other Names:
Nuclear receptor coactivator 2, Glucocorticoid receptor-interacting protein 1 (GRIP1)
Structure:
p160/steroid receptor coactivator family, nuclear localization sequence, bHLH, PAS domains; two isoforms 159 kD
Distribution:
Expressed in cytoplasm and translocated into nucleus upon phosphorylation. Highly expressed in placenta, skeletal muscle, uterus. Eexpressed in B cells, lymphocytes, and some tumors
Function:
Histone acetyltransferase activity, transcriptional coactivator for steroid and nuclear hormone receptors
Regulation:
Mediates ligand-independent nuclear translocation, activates CAR receptor in response to phenobarbital
Modification:
Phosphorylation
Interaction:
Forms a multisubunit coactivation complex with NCOA3, IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein, interacts with HIF1A, APEX, NR3C1, CAR
Antigen References:
1. Voegel J, et al. 1996. EMBO J. 15:3667. 2. Voegel J, et al. 1998. EMBO J. 17:507. 3. Hong H, et al. 1997. Mol. Cell. Biol. 17:2735.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-NCOA2/GRIP1
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-NCOA2/GRIP1 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
