This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
IF
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
Immunofluorescent microscope analysis of Hela cells using anti-NCOA1 polyclonal antibody (poly6087) (red). Alpha-tubulin (clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue).
Description:
NCOA1/SRC1 (nuclear receptor coactivator 1/steroid receptor coactivator 1) is a 157 kD member of the steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. Three isoforms of this nuclear protein have been reported. NCOA/SRC1 is expressed in astrocytoma, melanoma cell lines, lymphocytes, mammary gland, and skeletal muscle. This protein exhibits histone acetyltransferase activity, and is a transcriptional coactivator for steroid and nuclear hormone receptors. NCOA/SRC1 is inhibited by constitutively activated androstane receptor (CAR) signaling and activated by thyroid hormone receptor, and estrogen receptor ligation. NCOA/SRC1 is modified by phosphorylation and sumoylation; phosphorylation enhances coactivator function.This protein forms a complex with NCOA3, IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein, and has been shown to interacts with HIF1A, APEX, NR3C1, NR3C2, STAT6, STAT3, and the androgen receptor.
Histone acetyltransferase activity, transcriptional coactivator for steroid and nuclear hormone receptors
Regulation:
Inhibited by constitutively activated androstane receptor (CAR) signaling. Activated by thyroid hormone receptor, estrogen receptor ligation; phosphorylation enhances coactivator function
Modification:
Phosphorylation, Sumoylation
Interaction:
Forms a complex with NCOA3, IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein, interacts with HIF1A, APEX, NR3C1, NR3C2, STAT6, STAT3, androgen receptor
Antigen References:
1. Chauchereau, A., et al., 2003. J. Biol. Chem. 278:12335. 2. Min, G., et al., 2002. J. Biol. Chem. 277:34626. 3. Onate, S., et al., 1995. Science 270:1354.
