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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Immunofluorescence microscopy of Hela cells labeled with anti-NBS 1 (poly6138) (red). a-tubulin (clone 10D8) was also labeled (green); nuclei were stained with DAPI (blue). View Protocol
NBS1 (Nijmegen breakage syndrome 1, p95 protein of the MRE11/RAD50 complex) is a 95 kD nuclear protein that contains a FHA domain and BRCT domain. NBS1 is involved in DNA double-strand break repair, and DNA damage-induced checkpoint activation. NBS1 mutations are associated with Nijmegen breakage syndrome, characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. This protein is activated by irradiation, upregulated by c-Myc, and modified by phosphorylation. NBS1 is a component of the double-strand break repair complex consisting of DNL IV/XRCC4, Ku, MRE11, and RAD50. NBS1 has also been shown to associate with FANCD2. The Poly6138 antibody recognizes the C-terminal region of human and mouse NBS1 and has been shown to be useful for Western blotting.
Other Names:
Nijmegen breakage syndrome 1, p95 protein of the MRE11/RAD50 complex
Structure:
FHA domain, BRCT domain; 95 kD
Distribution:
Nuclear
Function:
DNA double-strand break repair, DNA damage-induced checkpoint activation. Mutations associated with Nijmegen breakage syndrome, characterized by microcephaly, growth retardation, immunodeficiency, cancer predisposition
Regulation:
Activated by irradiation, upregulated by c-Myc
Modification:
Phosphorylation
Interaction:
Part of double-strand break repair complex DNL IV/XRCC4, Ku, MRE11, RAD50; FANCD2
Antigen References:
1. Varon R, et al. 1998. Cell 93:467. 2. Huang J, et al. 2002. Nucleic Acids Res. 30:667. 3. Zhao S, et al. 2002. Nucleic Acids Res. 30:4815. 4. Chiang YC, et al. 2003. J. Biol. Chem. 278:19286.
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Purified anti-NBS1
Immunofluorescence microscopy of Hela cells labeled with anti-NBS 1 (poly6138) (red). a-tubulin (clone 10D8) was also labeled (green); nuclei were stained with DAPI (blue). View Protocol
