Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2 µg/ml antibody dilution. It is recommended that the reagent be titrated for optimal performance for each application.
Human stem cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with purified rabbit polyclonal anti-Nac1 antibody, proteins were visualized by HRP conjugated anti-rabbit secondary antibody and a chemiluminescence system.
Description:
Unlike other POZ/BTB family proteins, Nac1 lacks obvious DNA-binding domains in either of its two isoforms and acts as a transcriptional repressor. NAC1 is widely expressed in both neuronal and non-neuronal tissues. NAC1 protein is expressed at high levels in the brain and it plays an important role in behavioral sensitization, the histone deacetylase (HDAC) complex, and also interacts with other family members.
Other Names:
BTB(POZ) domain containing 14B
Structure:
NAC1 is a POZ/BTB motif containing transcriptional repressor protein and contains a conserved motif near the amino terminus that mediates protein–protein interactions like other POZ/BTB family proteins.
Distribution:
Nuclear, translocation to cytoplasm is activation dependent.
Function:
Mainly regulated by the Hedgehog pathway
Interaction:
BCL6 and CoREST.
Antigen References:
1. Cha, et al., 1997, J. Neurosci. 17, 6864 2. Korutla, et al., 2005 J. Neurochem. 94, 786
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.