Antibodies
|
Search   
Purified anti-Myosin heavy chain Antibody
Purified anti-Myosin heavy chain Antibody
621201 25 µl $85.00 Qty:
    
621202 100 µl $185.00 Qty:
    

Product Details

 
Clone: Poly6212 (See other available formats)
Isotype: Rabbit IgG
Reactivity: Human, Cross-Reactivity: Rat; reacts against 1960 aa length myosin heavy chain
Immunogen: Recombinant
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20°C.
Application:

IF - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Purified anti-Myosin heavy chain Antibody Poly6212 Image 1
Rat carotid artery frozen sections were stained with Poly6212, followed by anti-rabbit Alexa Fluor® 594 and DAPI staining.

Purified anti-Myosin heavy chain Antibody Poly6212 Image 2
Hela cells stained with purified rabbit polyclonal antibody against Myosin heavy chain (poly6212), followed by Cy3 conjugated Donkey anti-rabbit IgG and DAPI.


Compare all formats



Immunofluorescence Microscopy Protocol




Description:

Myosin heavy chain is an actin-based motor protein with an approximate molecular weight of 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment). This protein is highly expressed in muscle. Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion. Myosin heavy chain is modified by acetylation in cysteine residues in the S1 domain for myosin ATPase activity. Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits that forms filaments.

Other Names: Myosin heavy chain, muscle
Structure: Actin-based motor protein, approximately 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment)
Distribution: Highly expressed in muscle
Function: Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion
Regulation: Cysteine residues in the S1 domain are alkylated for myosin ATPase activity
Modification: Alkylation
Interaction: Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits. Forms filaments
Antigen
References:

1. Weiss A, et al. 1999. P. Natl. Acad. Sci. USA 96:2598.
2. Schiaffino S, et al. 1996. Physiol. Rev. 76:371 (review)

GeneID: 4627
Latest Publications: View the latest Myosin heavy chain articles on HighwirePress.com
UniProt: View information about Myosin heavy chain on UniProt.org
Keywords: Purified anti-Myosin heavy chain, Poly6212, Purified, Myosin heavy chain, muscle , Human, Cross-Reactivity: Rat; reacts against 1960 aa length myosin heavy chain, Immunofluorescence microscopy, Immunology, Antibodies


Product Reviews

  Title Rating Date
- There are currently no reviews for this product. Review this product at Biocompare.com to receive a gift card. -
      See more reviews using the Reviews Library


Product FAQs

 
There are no FAQs for this product. For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications
HRP Donkey anti-rabbit IgG (minimal x-reactivity)Poly4064ELISA, IHC, WB


Other Formats

DescriptionCloneApplications


Related Pathways

Pathway
Agranulocyte Adhesion and Diapedesis


Related Categories

Category
Cell Biology > Cell Motility/Cytoskeleton > Myosin Heavy Chain
Cell Biology > Antigens A-Z > Myosin Heavy Chain

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Myosin heavy chain
    Rat carotid artery frozen sections

    Rat carotid artery frozen sections were stained with Poly6212, followed by anti-rabbit Alexa Fluor® 594 and DAPI staining.

    Hela cells stained with purified

    Hela cells stained with purified rabbit polyclonal antibody against Myosin heavy chain (poly6212), followed by Cy3 conjugated Donkey anti-rabbit IgG and DAPI.

x
Copyright © 2013 BioLegend, Inc. All rights reserved.