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Purified Anti-mTOR Antibody
Material Safety Data Sheet (MSDS) Purified Anti-mTOR Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-mTOR Antibody
619901 50 µl (5 Western blots) $75.00     
619902 200 µl (20 Western blots) $225.00     
Clone: Poly6199
Isotype: Rabbit IgG
Reactivity: Human
Immunogen: Peptide corresponding to amino acids 2-18 of human mTOR
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20° C.
Application:

WB

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10-20 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application
References:

1. Wu, RF., et al. 2010. Mol Cell Biol. PubMed.

SK-N-MC cell extract was resolved

SK-N-MC cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-mTOR antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.


Description:

mTOR (mammalian target of rapamycin, FK506 binding protein 12-rapamycin associated protein 1 (FRAP1)) is a 289 kD member of the PI3 kinase family, containing, HEAT, FRB, FATC domains. mTOR is expressed in the nucleus and cytoplasm and is a target for cell-cycle arrest and the immunosuppressive effects of FKBP12-rapamycin complex. mTOR controls ribosome biogenesis, cell growth, and acts as an ATP sensor. mTOR is repressed by nutrient deprivation and mitochondrial uncoupling. Association of raptor negatively regulates mTOR kinase activity, also inhibited by tuberin-hamartin tumor suppressor complex. mTOR is modified by phosphorylation and associates with FKBP12, rapamycin, p70 S6 kinase, 4E-BP1, raptor, CLIP-170, tuberin, and hamartin. The Poly6199 antibody recognizes human mTOR and has been shown to be useful for Western blotting.

Other Names: Mammalian target of rapamycin, FK506 binding protein 12-rapamycin associated protein 1 (FRAP1)
Structure: PI3 kinase family, PI3/4 kinase, HEAT, FRB, FATC domains; 289 kD
Distribution: Nuclear, some cytoplasmic
Function: Target for cell-cycle arrest, immunosuppressive effects of FKBP12-rapamycin complex, controls ribosome biogenesis, cell growth, ATP sensor
Regulation: Repressed by nutrient deprivation and mitochondrial uncoupling. Association of raptor negatively regulates kinase activity, inhibited by tuberin-hamartin tumor suppressor complex
Modification: Phosphorylation
Interaction: FKBP12, rapamycin, p70 S6 kinase, 4E-BP1, raptor, CLIP-170, tuberin, hamartin
Antigen
References:

1. Brown, E., et al., 1994. Nature. 369:756.
2. Dennis, P., et al., 2001. Science. 294:1102.
3. Finga, D., et al., 2002. Genes Dev. 16:1472.
4. Nojima, H., et al., 2003. J. Biol. Chem. 278:15461.

HRP Donkey anti-rabbit IgG (minimal x-reactivity)Poly4064ELISA,IHC,WB
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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