ELISA: The purified 1F07-2C01 antibody is useful in a mouse VEGF-A ELISA or ELISPOT assay. Western Blotting: It is suggested to use 0.5-2 µg/ml for WB. Immunohistochemistry: Clone 1F07-2C01 is recommended for IHC on saponin-treated frozen tissue sections at a concentration range of 5-10 µg/ml.
It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
Reinders, M.E.J., et al., 2003. J. Clin. Invest. 112:1655.
Western blot analysis of recombinant mouse VEGF protein using anti-VEGF, clone 1F07-2C01.
Description:
VEGF-A, also known as VEGF (vascular endothelial growth factor), is the founding member of the family including VEGF (VEGF-A), VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides with a highly conserved receptor-binding cystine-knot structure similar to that of the platelet-derived growth factors.VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis.VEGF-A is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. Oxygen tension is a key physiological regulator of VEGF-A gene expression.
Other Names:
Vascular endothelial growth factor (VEGF), VEGF-A
Structure:
cystine-knot growth factors
Distribution:
aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells
Function:
angiogenesis
Regulation:
HIF-1, hypoxiaresponsiveness
Cellular Targets:
endothelial cells
Receptors:
VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1)
Antigen References:
1. Contreras, A., et al., 2007. Am. J. Transpl. 7:155. 2. Lambrechts, D. and Carmeliet, P., 2007. Biochim. Biophys. Acta. 1762:1109. 3. Tong, J.P. and Yao, Y.F., 2006. Clin. Biochem. 39: 267. 4. Reinders, M.E.J., et al., 2003. J. Clin. Invest. 112:1655 5. Ferrara, N.,et al., 2003. Nat. Med. 9:669. 6. Shibuya, M., 2001. Cell Struct. Funct. 26:25. 7. Senger, D.R., et al., 1983. Science. 219:983.
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