Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.
Application References:
1. Nakae, S., et al., 2007. Blood doi:10.1182/blood-2006-11-058800.
Mouse Tim-3 transfected cells stained with purified RMT3-23, followed by anti-rat IgG FITC
Description:
Tim-3 is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
Other Names:
T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2
Structure:
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD. Can exist as a soluble form lacking mucin and transmembrane domains
Distribution:
Expressed on activated Th1 lymphocytes and CD11b+ macrophages
Function:
May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand Receptor:
Putative ligand on resting CD4+ lymphocytes
Antigen References:
1. Sabatos, C. A., et al., 2003. Nat. Immunol. 4:1102. 2. Sanchez-Fueyo, A., et al., 2003. Nat. Immunol. 4:1102. 3. Kuchroo, V.K., et al., 2003. Nat. Rev. Immunol. 3:454. 4. Mooney, L., et al., 2002. Nature 415:536.
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