The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.
Application References:
1. Nakae, S., et al., 2007. Blood doi:10.1182/blood-2006-11-058800.
Description:
Tim-2 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-2. It is expressed on activated T cells and preferentially on differentiated Th2 cells. Sema4A is the ligand of Tim-2. A blockade of Tim-2/Tim-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. Tim-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.
Other Names:
T cell immunoglobulin and mucin domain containing protein-2
Structure:
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 33 kD
Distribution:
Developmentally regulated; highly expressed in embryonic development at the 8-cell stage and blastocyst. Expressed on activated T lymphocytes.
Function:
Plays a critical role in immune responses and the development of atopic diseases
Ligand Receptor:
Binds to Sema4A
Antigen References:
1. Kuchroo, V.K., et al. 2003. Nat. Rev. Immunol. 3:454. 2. Kumanogoh, A., et al. 2002. Nature 419:629. 3. Chakravarti, S., et al. 2005. J. Exp. Med. 202:437.
