Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.
Application References:
1. Meyers, J.H., et al., 2005. Nat. Immunol. 6:455. 2. Nakae, S., et al., 2007. Blood doi:10.1182/blood-2006-11-058800. 3. Yamanishi, Y. et. al., 2010. J. Exp. Med. 207:1501. (FC)
Mouse Tim-1 transfected cells stained with purified RMT1-4, followed by anti-rat IgG FITC
Description:
Tim-1 is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-1 and hepatitis virus cellular receptor 1. It is developmentally expressed at high levels in the blastocyst. Tim-1 is expressed on activated CD4+ lymphocytes especially on Th2 cells and has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in costimulation of T cell proliferation. Tim-1 is an endogenous ligand for LMIR5/CD300b.
Other Names:
T cell immunoglobulin and mucin domain containing protein-1, T cell and airway phenotype regulator (Tapr), hepatitic virus cellular receptor 1
Structure:
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 33 kD.
Distribution:
Developmentally regulated; highly expressed in embryonic development in blastocysts. Expressed on activated CD4+ lymphocytes, especially Th2 cell lines.
Function:
Binds hepatitis virus, may play a critical role in immune responses and the development of atopic diseases (in particular airway hyperreactivity).
Ligand Receptor:
Binds hepatitis A virus in humans, Tim-4, LMIR5/CD300b
Antigen References:
1. McIntire, J. J., et al., 2001. Nature Immunol. 2:1109. 2. Kuchroo, V.K., et al., 2003. Nat. Rev. Immunol. 3:454. 3. Wills-Karp, M., et al., 2001. Nat. Rev. Immunol. 1:69. 4. Meyers, J.H., et al., 2005. Nat. Immunol. 6(5):455.
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