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Each lot of this antibody is quality control tested by ELISA analysis. For ELISA capture applications, a concentration range of 5-10 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 μg per 106 cells in 100 μl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA or ELISPOT Capture1: The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization1,4,6: The LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 505906). Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry5 of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Application References:
1. Luo, Y., et al. 1994. J. Immunol. 153:3708. 2. Zhang, Y., et al. 2002. J. Immunol. 168:3088. 3. Luo, Y., et al. 1999 J. Immunol. 163:3985. 4. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862. 5. Hancock, W. W., et al. 1997 Transplantation 64:696. 6. Yu, R., et al. 2006 Obesity 14:1353. 7. Patsouris, D., et al. 2009. J. Biol Chem. 284:31223. PubMed 8. Rutar, M., et al. 2011. Invest Ophtalmol Vis Sci. 52:5379. PubMed.
Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.
Other Names:
Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2,
Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors:
CCR2; CCR5; CCR10; D6; possibly L-CCR
Bioactivity/Activities:
Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Antigen References:
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271. 3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752. 4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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PE anti-mouse/rat/human MCP-1
LPS-stimulated human peripheral blood monocytes were surface stained with CD14 FITC and then intracellular stained with 2H5 PE
