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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,5, in vivo receptor blocking1-2,4-6,8, and in vitro neutralization7. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115804) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 115808).
Application References:
1. Coulie PG, et al. 1990. Curr. Top. Microbiol. Immunol. 166:43. (Block, IP) 2. Vink A, et al. 1990. J. Exp. Med. 172:997. (Block, IP) 3. Sun R, et al. 2004. J. Immunol. 172:5648. (FC) 4. McKinney WJ, et al. 1998. Am. J. Respir. Cell Mol. Biol. 18:696. (Block) 5. Weigert C, et al. 2006. J. Biol. Chem. 281:7060. (Block, IP) 6. Koizumi N, et al. 2007. J. Immunol. 178:1767. (Block) 7. Walker F, et al. 2008. Blood 111:3978. (Neut) PubMed 8. Yadav M, et al. 2008. J. Immunol. 180:2772. (Block) PubMed 9. Sintes J, et al. 2010. J. Leukoc. Biol. 88:687. PubMed
C57BL/6 mouse splenocytes stained with purified D7715A7, followed by biotinylated anti-rat IgG and Sav-PE
CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. The D7715A7 (15A7) antibody blocks IL-6/IL-6 receptor interactions.
Other Names:
IL-6 Receptor α chain, IL-6R
Structure:
Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution:
Activated T and B cells, monocytes, hepatocytes, plasma cells
Function:
Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand Receptor:
IL-6
Antigen References:
1. Taga T, et al. 1997. Annu. Rev. Immunol. 15:797. 2. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London. 3. Boulanger MJ, et al. 2003. Science 300:2101.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse/rat CD126 (IL-6Rα chain)
F0 mouse myeloma cell line stained with D7715A7 PE
