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For immunohistochemical staining, the suggested use of this reagent is ≤ 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking1-4 of cell adhesion, immunohistochemical staining1,2,6 of acetone-fixed frozen sections and 4% paraformaldehyde-fixed paraffin-embedded sections, immunoprecipitation3,5, and Western blotting6.
Application References:
1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853. (IHC, Block) 2. Michie SA, et al. 1993. Amer. J. Path. 143:1688. (IHC, Block) 3. Berg EL, et al. 1991. J. Cell Biol. 114:343. (IP, Block) 4. Berg EL, et al. 1993. Nature 366:695. (Block) 5. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219. (IP) 6. Samulowitz U, et al. 2002. Am. J. Path. 160:1669. (IHC, WB) 7. Burke SD, et al. 2007. Diabetes 56:2919. PubMed 8. Hirakawa J, et al. 2010. J. Biol. Chem. 285:40864. PubMed
Description:
MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 β1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.
Other Names:
PNAd, Peripheral Node Addressin, MECA-79
Structure:
Sulphated CD34, GlyCAM-1 and MAdCAM-1, Sgp200, sialomucin
Distribution:
High endothelial venules (HEV) in lymphoid tissues, inflamed tissues and rheumatoid synovia
Function:
Leukocyte homing, rolling
Ligand Receptor:
CD62L
Antigen References:
1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853. 2. Berg EL, et al. 1993. Nature 366:695. 3. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219. 4. Mitoma J, et al. 2003. J. Biol. Chem. 278:9953. 5. Bruehl RE, et al. 2000. J. Biol. Chem. 275:32642. 6. Bistrup A, et al. 2004. Am. J. Pathol. 164:1635
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