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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.
C57BL/6 mouse splenocytes were stained with CD8 APC and purified PD-1H (clone MH5A) (top) or Armenian Hamster IgG isotype control (bottom), followed by anti-Armenian Hamster IgG-PE.
PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants.
Other Names:
PD-1 homolog, Dies1
Structure:
Type I transmembrane protein, member of the CD28 family
Distribution:
Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells
Function:
Negative regulator of the immune response
Antigen References:
1. Flies DB, et al. 2011. J. Immunol. 187:1537. 2. Wang Li, et al. 2011. J. Exp Med. 208:577.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 APC and purified PD-1H (clone MH5A) (top) or Armenian Hamster IgG isotype control (bottom), followed by anti-Armenian Hamster IgG-PE.
PE anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 APC and PD-1H (clone MH5A) PE (top) or Armenian Hamster IgG PE isotype control (bottom).
LEAF™ Purified anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 APC and purified PD-1H (clone MH5A) (top) or Armenian Hamster IgG isotype control (bottom), followed by anti-Armenian Hamster IgG-PE.
FITC anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 PE and PD-1H (clone MH5A) FITC (top) or Armenian Hamster IgG FITC isotype control (bottom).
APC anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-1H (clone MH5A) APC (top) or Armenian hamster IgG APC isotype control (bottom).
PerCP/Cy5.5 anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-1H (clone MH5A) PerCP/Cy5.5 (top) or Armenian hamster IgG PerCP/Cy5.5 isotype control (bottom).
