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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing1-4,7, immunohistochemical staining1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting1.
Application References:
1. Streeter PR, et al. 1988. Nature 331:41. 2. Briskin MJ, et al. 1993. Nature 363:461. 3. Berlin C, et al. 1993. Cell 74:185. 4. Bargatze RF, et al. 1995. Immunity 3:99. 5. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581. 6. Savinov AY, et al. 2003. J. Exp. Med. 197:643. 7. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.
TNF-a-stimulated bEND.3 cells stained with MECA-367 biotin, followed by Sav-PE
MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin α4β7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. The MECA-367 antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of the mAb is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.
Other Names:
Mucosal addressin cell adhesion molecule-1
Structure:
Type I glycoprotein, Ig superfamily
Distribution:
HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas
Function:
Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand Receptor:
Integrin a4ß7, CD62L
Antigen References:
1. Streeter PR, et al. 1988. Nature 331:41 2. Briskin MJ, et al. 1993.Nature 363:461. 3. Berlin C, et al. 1993. Cell 74:185. 4. Lehnert K, et al. 1998. Eur. J. Immunol. 28:3605. 5. Picarella D, et al. 1997. J. Immunol. 158:2099.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse MAdCAM-1
TNF-a-stimulated bEND.3 cells stained with MECA-367 biotin, followed by Sav-PE
Biotin anti-mouse MAdCAM-1
TNF-a-stimulated bEND.3 cells stained with MECA-367 biotin, followed by Sav-PE
