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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1.
Application References:
1. Nagasawa R, et al. 1987. J. Immunol. 138:815. (IP) 2. Guy-Grand D, et al. 1996. Eur. J. Immunol. 26:2248. (FC) 3. Zimmer J, et al. 2001. J. Exp. Med. 194:1531. (FC)
C57BL/6 mouse splenocytes stained with purified A1/Ly49A conjugated with PE and NK1.1 APC
Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2Dd and H-2Dk, triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The A1/Ly49A antibody preferentially reacts with CRD of Ly-49AB6 alloantigen and has also shown cross-reaction with Ly-49ANOD, Ly-49PNOD and Ly-49P129/J.
Other Names:
Ly49A
Structure:
C-type lectin, Ly-49 family, ITIM, 44 kD
Distribution:
NK cells, NKT cells
Function:
Inhibitory NK cell receptor
Ligand Receptor:
H-2Dd, H-2Dk, H-2Dp
Antigen References:
1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236. 2. Takei FJ, et al. 1997. Immunol. Rev. 155:67. 3. Hanke T, et al. 1999. Immunity 11:67. 4. Silver ET, et al. 2000. J. Immunol. 165:1771.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse Ly-49AB6
C57BL/6 mouse splenocytes stained with A1/Ly49A PE and NK1.1 APC
Purified anti-mouse Ly-49AB6
C57BL/6 mouse splenocytes stained with purified A1/Ly49A conjugated with PE and NK1.1 APC
