Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2
.
Application References:
1. Takei F. 1983. J. Immunol. 130:2794. 2. Chan P, et al. 1989. J. Immunol. 142:1727.
C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC
Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2Dd and H-2Dk, triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The YE1/48.10.6 antibody reacts with NK cells from all mouse strains tested and reverses the inhibition of NK cytotoxicity.
Structure:
C-type lectin, Ly-49, ITIM, 44 kD
Distribution:
NK and NK-T cells
Function:
Inhibitory NK cell receptor
Ligand Receptor:
MHC Class I Molecules
Antigen References:
1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236. 2. Takei FJ, et al. 1997. Immunol. Rev. 155:67. 3. Hanke T, et al. 1999. Immunity 11:67.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse Ly-49A
C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC
FITC anti-mouse Ly-49A
C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC
PE anti-mouse Ly-49A
C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC
Purified anti-mouse Ly-49A
C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC
Pacific Blue™ anti-mouse Ly-49A
C57BL/6 mouse splenocytes stained with anti-Ly49G FITC and YE1/48.10.6 Pacific Blue™
