The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C.
Application:
FC - Quality Tested IP4*, IHC(Frozen)5 * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking1-4 of cell adhesion, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining5 of frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 120604).
Application References:
1. Andrew, D. P. et al. 1994. J. Immunol. 153:3847. 2. Berlin, C. et al., 1993. Cell 74:185. 3. Berlin-Rufenach , C. et al., 1999. J. Exp. Med. 189:1467. 4. Rivera-Nieves, J., et al., 2005. J. Immunol. 174:2343. 5. Bogetto, L., et al., 2000. Blood 95:2397.
C57BL/6 mouse splenocytes stained with purified DATK32, followed by anti-rat IgG FITC
Description:
DATK32 antibody is specific for a combinatorial determinate of integrin α4β7complex. Integrin α4β7 is composed of a 150 kD (α4 or CD49d) and a 130 kD (β7) heterodimer, also known as CD49d/β7, or LPAM-1. It belongs to Ig superfamily found on majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin α4β7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and helps direct the migration of blood lymphocytes to the intestine and associated lymphoid tissues. It has been reported that the DATK32 mAb is able to in vitro and in vivo block α4β7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1 and fibronectin
Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells
Function:
Lymphocyte adhesion
Ligand Receptor:
VCAM-1 (CD106), MAdCAM-1, fibronectin
Antigen References:
1. Andrew, D. P., et al., 1994. J. Immunol. 153: 3847. 2. Berlin, C., et al., 1994. Cell 74: 185. 3. Gurish, M. F., et al., 2001 J. Exp. Med. 194: 1243. 4. Hamann, A., et al., 1994. J. Immunol. 152: 3282.
