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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg/ml dilution buffer for each mini-gel.
COA:
Application Notes:
Clone BL35175 has been shown to be useful for detecting mouse and human IL-22 by Western blotting.
20 ng recombinant mouse Il-22 protein was separated by electrophoresis, transferred to nitrocellulose, and probed with monoclonal mIL-22 antibody (BL35175). Protein was visualized using a goat anti-rat IgG secondary conjugated to HRP and chemiluminescence detection.
IL-22, a cytokine structurally related to IL-10, was originally identified in the mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as IL-TIF (IL10-related T cell-derived inducible factor). Mouse IL-TIF consists of 179 amino acids, including four cysteins, and has a predicted molecular weight of 20 kD. Biological activity of IL-22 is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor families. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on non-immune cells to regulate local tissue inflammation. As a product of the recently identified T helper 17 lineage of CD4(+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in dysregulated inflammation observed in autoimmune diseases.
179 aa with with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein.
Distribution:
Secreted, Th17
Function:
Cytokine that contributes to the inflammatory response in vivo.
Ligand Receptor:
Binds to IL-22 receptor complex that consists of two receptor subunits, IL22R and IL10Rβ.
Antigen References:
1. Dumoutier L, et al. 2000. J. Immunol. 164:1814. 2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335. 3. Jones BC, et al. 2008. Structure 16:1333. 4. Sonnenberg GF, et al. 2010. J. Exp. Med. 207:1293. 5. Kotenko SV, et al. 2001. J. Immunol. 166:7096. 6. Chang C, et al. 2006. Cell Research 16:902.
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Purified anti-mouse IL-22
20 ng recombinant mouse Il-22 protein was separated by electrophoresis, transferred to nitrocellulose, and probed with monoclonal mIL-22 antibody (BL35175). Protein was visualized using a goat anti-rat IgG secondary conjugated to HRP and chemiluminescence detection.
