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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3 of acetone-fixed frozen sections.
Application References:
1. Nitschke, L., et al. 1993. P. Natl. Acad. Sci. 90:1887. (FC) 2. Weih, D., et al. 2001. J. Immunol. 167:1909. (IHC) 3. Koni, P.A., et al. 2001. J. Exp. Med. 193:741. (IHC) 4. Ahuja, A., et al.. 2007. J. Immunol. 179:3351. (FC) PubMed 5. Haynes, N.M., et al. 2007. J. Immunol. 179:5099. (FC) 6. Good-Jacobson, K.L., et al. 2010. Nat. Immunol. 11:535. (FC) PubMed 7. Tomyako, M.M., et al. 2010. J. Immunol. 185:7146. PubMed
C57BL/6 mouse splenocytes stained with purified 11-26c.2a, followed by anti-rat IgG FITC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse IgD
C57BL/6 mouse splenocytes stained with 11-26c.2a FITC
PE anti-mouse IgD
C57BL/6 splenocytes stained with
11-26c.2a PE
Purified anti-mouse IgD
C57BL/6 mouse splenocytes stained with purified 11-26c.2a, followed by anti-rat IgG FITC
Alexa Fluor® 647 anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647
PerCP/Cy5.5 anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a PerCP/Cy5.5
Pacific Blue™ anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a Pacific Blue™ and CD19 antibody (6D5) PE
C57BL/6 splenocytes stained with rat IgG2a Pacific Blue™ isotype and CD19 antibody (6D5) PE
APC anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a APC
APC/Cy7 anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a APC/Cy7
Alexa Fluor® 488 anti-mouse IgD
C57BL/6 splenocytes were stained with B220 APC and IgD (11-26c.2a) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
