Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunohistochemical staining of frozen sections, the suggested use of this reagent is 4 to 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen sections1 and formalin fixed paraffin-embedded sections3, Western blotting2,3,4.
Application References:
1. Harrington, L., et al., 2008. Infect. Immunity 76:3045. (IHC) PubMed 2. Dai, X., et al., 2009. J. Histochem. Cytochem. 58:17. (WB) PubMed 3. Sorrentino, R., et al., 2009. Am. J. Resp. Cell Mol. Epub. (WB, IHC)PubMed 4. Muller, A.J., et al., 2010. Cancer Res. 70:1845. (WB) PubMed
Mouse macrophage extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal IDO (Clone mIDO-48) antibody. Proteins were visualized using a goat anti-rat secondary conjugated to HRP and a chemiluminescence detection system.
Description:
The mIDO-48 monoclonal antibody recognizes mouse IDO also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenas. This antibody does not appear to react with human IDO. IDO is a ubiquitously expressed cytoplasm protein with a predicted molecular weight approximately 45 kD. Indoleamine 2,3-dioxygenase (IDO) is one the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated in protection against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, kidney, and placenta. The IDO antibody has been shown to be useful for western blotting and Immunohistochemistry.
IDO is a monomeric haemoprotein, predicted molecular weight approximately 45 kDa.
Distribution:
Cytoplasm, ubiquitously expressed
Function:
Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated in protection against intracellular and extracellular pathogens.
Antigen References:
1. Habara-Ohkubo A., et al. 1991.Gene 105:221. 2. Munn, D.H. et al. 2002. Science 297:1867.
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