This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:
The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C.
Application:
WB - Quality Tested IHC(Frozen)1*, IF* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunohistochemical staining of frozen sections, the suggested use of this reagent is 4 to 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Harrington, L. et al. 2008. Infect Immunity 76:3045. PubMed 2. Dai, X., et al. 2009. J. Histochem Cytochem. PubMed 3. Sorrentino, R., et al. 2009. Am J. Respir Cell Mol Biol. PubMed.
Mouse macrophage extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal IDO (Clone mIDO-48) antibody. Proteins were visualized using a goat anti-rat secondary conjugated to HRP and a chemiluminescence detection system.
Description:
The mIDO-48 monoclonal antibody recognizes mouse IDO also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenas. This antibody does not appear to react with human IDO. IDO is a ubiquitously expressed cytoplasm protein with a predicted molecular weight approximately 45 kD. Indoleamine 2,3-dioxygenase (IDO) is one the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated in protection against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, Kinney, and placenta. The IDO antibody has been shown to be useful for western blotting and Immunohistochemistry.
IDO is a monomeric haemoprotein, predicted molecular weight approximately 45 kDa.
Distribution:
Cytoplasm, ubiquitously expressed
Function:
Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated in protection against intracellular and extracellular pathogens.
Antigen References:
1. Habara-Ohkubo A., et al. 1991.Gene 105:221. 2. Munn, D.H. et al. 2002. Science 297:1867.
