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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens. It cross-reacts with the H-2p,r haplotypes. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 107610).
Application References:
1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP) 2. Viville S, et al. 1993. Cell 72:635. (IHC) 3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC) 4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block) 5. Yamashita I, et al. 1993. Int. Immunol. 5:1139. 6. Guo M, et al. 1995. Zygote 3:65. (IHC) 7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block) 8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177. 9. Venanzi ES, et al. 2007. J. Immunol. 179:5693. 10. Christensen SR, et al. 2006. Immunity 25:417. PubMed 11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed 12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed 13. Kuns RD, et al. 2009. Blood 113:5999. PubMed 14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed 15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed.
C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
Other Names:
MHC class II
Structure:
MHC class II
Distribution:
B and activated T cells, APCs of the H-2b,d,q,r bearing mice
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD4
Antigen References:
1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Purified anti-mouse I-A/I-E, M5/114.15.2, Purified, MHC class II, Mouse, Reacts with a shared determinant on I-Ab,d,q, and I-Ed,k alloantigens., Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or biotinylated rat IgG2b, κ isotype control (dashed line) followed by Sav-PE.
FITC anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
PE anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE (solid line) or rat IgG2b, κ PE isotype control (dashed line).
Purified anti-mouse I-A/I-E
C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
LEAF™ Purified anti-mouse I-A/I-E
C57/B6 mouse splenocytes were stained with LEAF™ anti-mouse I-A/I-E (clone M5/114.15.2) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by IgG-FITC.
PE/Cy5 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE/Cy5 (filled histogram) or rat IgG2b, κ PE/Cy5 isotype control (open histogram).
APC anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2)APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
Alexa Fluor® 488 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) Alexa Fluor® 488 (filled histogram) or rat IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).
Alexa Fluor® 647 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) Alexa Fluor® 647 (filled histogram) or rat IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
Pacific Blue™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram).
Alexa Fluor® 700 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2)Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram).
PerCP/Cy5.5 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PerC/Cy5.5P (filled histogram) or rat IgG2b, κ PerCP/Cy5.5 isotype control (open histogram).
PerCP anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PerCP (filled histogram) or rat IgG2b, κ PerCP isotype control (open histogram).
APC/Cy7 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) APC/Cy7 (filled histogram) or rat IgG2b, κ APC/Cy7 isotype control (open histogram).
PE/Cy7 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE/Cy7 (orange line) or rat IgG2b, κ PE/Cy7 isotype control (purple line).
Brilliant Violet 421™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (clone M5/114.15.2) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
