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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For Western blotting, the suggested working dilution is 0.5-1.0 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Galectin-3 will work for either FC or ICFC applications depending on whether it is on the surface or located intracellularly.
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-galectin-3 antibody (clone Gal397). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
Galectin-3 (galactose-specific lectin 3) is a 30-32 kD glycoprotein, also know as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and εBP. It is a member of animal β-galactoside-binding protein family that is expressed in cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3contains carbohydrate recognition domains and binds to β-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Galectin-3 structurely possesses NWGR motif that is conserved in BH-1 domain of BCL-2 family and functions as an anti-apoptotic molecule.
30-32 kD glycoprotein, animal β-galactoside-binding protein family
Distribution:
Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, dendritic cells
Function:
Regulate cell proliferation and differentiation, adhesion, anti-apoptosis
Ligand Receptor:
Variety ligands, such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, etc
Antigen References:
1. Kim HRC, et al. 1999. Cancer Res. 59:4148 2. Reljic R, et al. 2004. Immunol. Lett. 93:51. 3. Takenaka Y et al. 2004. Mol. and Cell. Biol. 24:4395 4. Joo GH, et al. 2001. J. Leukoc. Biol. 69:555
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse/human Galectin-3 (Mac-2)
Human peripheral blood monocytes intracellular stained with Gal397 PE
Purified anti-mouse/human Galectin-3 (Mac-2)
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-galectin-3 antibody (clone Gal397). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
