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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2,3, blocking of cell adhesion4,5, and immunohistochemical staining of acetone-fixed frozen sections6.
The 34-2-12 antibody recognizes α3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. Clone 34-2-12 recognizes the α3 domain of Dd.
Other Names:
MHC class I
Structure:
MHC class I
Distribution:
Nucleated cells of the H-2Dd haplotype
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD8
Antigen References:
1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Purified anti-mouse H-2Dd, 34-2-12, Purified, MHC class I, Mouse d haplotype, Cross-reacts with the C3H.LG/Ckc. Does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s)., Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse H-2Dd
BALB/c mouse splenocytes stained with 34-2-12 PE
C3H/He mouse splenocytes stained with 34-2-12 PE
FITC anti-mouse H-2D d
BALB/c mouse splenocytes stained with 34-2-12 PE
C3H/He mouse splenocytes stained with 34-2-12 PE
PE anti-mouse H-2D d
BALB/c mouse splenocytes stained with 34-2-12 PE
C3H/He mouse splenocytes stained with 34-2-12 PE
Purified anti-mouse H-2Dd
BALB/c mouse splenocytes stained with 34-2-12 PE
C3H/He mouse splenocytes stained with 34-2-12 PE
Alexa Fluor® 647 anti-mouse H-2D d
BALB/c mouse splenocytes stained with 34-2-12 Alexa Fluor® 647
