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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
C57BL/6 mouse platelets were stained with purified GARP (clone F011-5) (filled histogram) or rat IgG2a, κ (open histogram), followed by anti-rat IgG PE.
Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing protein 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs. It serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.
Other Names:
LRRC32, Garpin, Glycoprotein A repetitions predominant
Structure:
80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution:
Activate Tregs, megakaryocytes, platelets
Ligand Receptor:
LAP (TGF-β1)
Antigen References:
1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213. 2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315. 3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439. 4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse GARP (LRRC32)
C57BL/6 mouse platelets were stained with purified GARP (clone F011-5) (filled histogram) or rat IgG2a, κ (open histogram), followed by anti-rat IgG PE.
PE anti-mouse GARP (LRRC32)
CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes (48 hours) were surface stained with CD4 FITC, and GARP (clone F011-5) PE (top) or rat IgG2a, κ PE isotype control (bottom), then intracellularly stained with FOXP3 Alexa Fluor® 647. Data shown was generated by gating on the CD4+ lymphocyte population.
