This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by affinity chromatography.
Storage & Handling:
Store undiluted at 4°C. Do not freeze.
Application:
FC - Quality Tested FA, IHC* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0µg/test for FC. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Moriyama Y et al. 2008. Int J Immunol 20:763 (FC)
FD1/CHO (mouse DLL1 transfected cel l line) stained with purified HMD1-3, followed by anti-Armenian hamster IgG PE
Description:
The Notch receptors and their ligands are highly conserved from invertebrates to mammals.The Delta-like 1 (Dll1) is one of the four or five Notch ligandsidentified.The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus.This translocation triggers a series of signaling process.Delta-like 1 is reported to be essential for the maintenance of marginal zone B cells in normal mice and engagement of Notch 1 by Dll 1 promotesdifferentiation of B lymphocytes to antibody-secreting cells.
Other Names:
Dll 1, Dll-1
Structure:
A single pass transmembrane protein consisting of a large extracellular N-terminal which includes the DSL domain, followed by a tandem array of EGF-like repeats (ELRs). After a single transmembrane domain (TM) is a novel intracellular domain.
Function:
The Notch receptors and their ligands are highly conserved signaling molecules throughout development. They regulate cell fate decision for various cell development, including myogenesis, neurogenesis, and lymphocyte development.
