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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 116110).
Mouse splenocytes extract was resolved by electrophoresis under non-reducing condition, transferred to nitrocellulose and probed with monoclonal anti-mouse ICAM-1 (Clone YN1/1.7.4) antibody. Proteins were visualized using a goat anti-rat secondary conjugated to HRP and a chemiluminescence detection system.
C57BL/6 mouse splenocytes stained with purifid YN1/1.7.4 , followed by anti-rat IgG FITC
CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.
Other Names:
ICAM-1, Ly-47
Structure:
Ig superfamily, 90 kD
Distribution:
Activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, dendritic cells
Function:
Immune reaction, inflammation, adhesion
Ligand Receptor:
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD54
Mouse splenocytes extract was resolved by electrophoresis under non-reducing condition, transferred to nitrocellulose and probed with monoclonal anti-mouse ICAM-1 (Clone YN1/1.7.4) antibody. Proteins were visualized using a goat anti-rat secondary conjugated to HRP and a chemiluminescence detection system.
C57BL/6 mouse splenocytes stained with purifid YN1/1.7.4 , followed by anti-rat IgG FITC
Biotin anti-mouse CD54
C57BL/6 mouse splenocytes stained with biotinylated YN1/1.7.4 , followed by Sav-PE
FITC anti-mouse CD54
C57BL/6 splenocytes stained with YN1/1.7.4 FITC
PE anti-mouse CD54
C57BL/6 mouse splenocytes stained with YN1/1.7.4 PE
LEAF™ Purified anti-mouse CD54
C57BL/6 mouse splenocytes stained with LEAF™ purified YN1/1.7.4 , followed by anti-rat IgG FITC
Alexa Fluor® 488 anti-mouse CD54
C57BL/6 splenocytes stained with YN1/1.7.4 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD54
C57BL/6 mouse splenocytes stained with YN1/1.7.4 Alexa Fluor® 647
Pacific Blue™ anti-mouse CD54
C57BL/6 mouse splenocytes stained with YN1/1.7.4 Pacific Blue™
