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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
Wang H, et al. 2007. P. Natl. Acad. Sci. USA 104:13744
C57BL/6 splenocytes stained with miap301 purified, followed by anti-rat IgG FITC
CD47, also known as Integrin-Associated Protein (IAP) ,is a membrane protein of about 50 kd with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region.It is widely expressed on many cell types and often associated with beta 3 integrins.The significance of this molecules is recently drawing increasing attention.It has been reported that CD47 functions as a self marker.Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages.By binding to SIRPalpha,CD47 controls hemostatic innate immune functions,such as phagocytosis and cell trafficking.
Other Names:
Integrin-associated protein, IAP, neurophilin,gp42, Rh-associated protein
Structure:
50kd plasma membrane protein of immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids and a short cytoplasmic tail of 30 amino acids.
Distribution:
Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.
Function:
Adhesion molecules, immune regulation
Ligand Receptor:
SIRPalpha (CD172alpha, Signal regulatory protein alpha), thrombospondin
Interaction:
Associate with beta3 integrins
Bioactivity/Activities:
Upon interaction with SIRPalpha, controls innate immune effector function, such as host cell phagocytosis
Antigen References:
1. Brown E, et al. 1990. J Cell Biol 111:2785 2. Mawby WJ, et al.<.i> 1994. Biochem J 304:525 3. Gao AG, et al. 1996. J. Biol. Chem. 271:21 4. Oldenborg PA, et al. 2000. 288:2051 5. van Beck EM, et al. 2005. J. Immunol. 175:7781
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD47
C57BL/6 splenocytes stained with miap301 purified, followed by anti-rat IgG FITC
FITC anti-mouse CD47
Balb/c mouse splenocytes stained with Miap301 FITC
Biotin anti-mouse CD47
BALB/c splenocytes stained with Miap301 biotin, followed by Sav-PE
PE anti-mouse CD47
Balb/c mouse splenocytes stained with Miap301 PE
Alexa Fluor® 647 anti-mouse CD47
BALB/c mouse splenocytes stained with Miap301 Alexa Fluor® 647
