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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
Application References:
1. Bottomly K, et al. 1989. Eur. J. Immunol. 19:617. 2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
C57BL/6 mouse splenocytes stained with purified C363-16A, followed by anti-rat IgG FITC
CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4+ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.
Structure:
Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution:
Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells
Function:
Phosphatase, T cell development and activation
Ligand Receptor:
Galectin-1, CD2, CD3, CD4, Thy1
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD45RB
C57BL/6 mouse splenocytes stained with C363-16A FITC
PE anti-mouse CD45RB
C57BL/6 splenocytes stained with
C363-16A PE
Purified anti-mouse CD45RB
C57BL/6 mouse splenocytes stained with purified C363-16A, followed by anti-rat IgG FITC
APC/Cy7 anti-mouse CD45RB
C57BL/6 mouse splenocytes stained with C363-16A APC/Cy7
Alexa Fluor® 647 anti-mouse CD45RB
C57Bl/6 mouse splenocytes stained with C363-16A Alexa Fluor® 647
PerCP/Cy5.5 anti-mouse CD45RB
C57BL/6 mouse splenocytes stained with C363-16A PerCP/Cy5.5
Pacific Blue™ anti-mouse CD45RB
C57BL/6 splenoctyes stained with C363-16A Pacific Blue™
