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Purified anti-mouse CD309 (VEGFR2, Flk-1) Antibody
Purified anti-mouse CD309 (VEGFR2, Flk-1) Antibody
136401 50 µg ¥12,000     
136402 500 µg ¥43,000     

Product Details

Clone: Avas12
Isotype: Rat IgG2a, κ
Isotype Control:Purified Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: Murine Flk1 fused to hIgG Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4 °C.
Application:

FC - Quality tested
WB, IHC - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
References:

1. Kataoka H, et al. 1997. Develop. Growth Diff. 39:729

Endothelial bEnd.3 cells stained with
Endothelial bEnd.3 cells stained with Avas 12 PE


Compare all formats



Description:

CD309, also known as vascular endothelial growth factor receptor 2, VEGFR2, fetal liver kinase-1. CD309 is a member of the tyrosine protein kinase family that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. FLK-1 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle. FLK-1 is a receptor for vascular endothelial growth factor (VEGF) or VEGFC. It plays an important role in the development of vascular endothelial, hematopoietic cells, and vascular permeability. Avas 12 recognizes different epitope from clone 89B3A5.

Other Names: Flk-1, VEGF receptor-2, VEGF-R2, Ly-73, fetal liver kinase 1
Structure: A receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF receptor α chain) of the immunoglobulin superfamily.
Distribution: Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle.
Function: Plays an important role in the development of vascular endothelial, hematopoietic cells, and vascular permeability.
Ligand Receptor: Vascular endothelial growth factor (VEGF)
Antigen
References:

1. Kaburn N, et al. 1997. Development. 124:2039
2. Patterson C, et al. 1995. J. Bio. Chem. 270:23111
3. Nishikawa SI, et al. 1998. Immunity 8 (6):761
4. Shalaby F, et al. 1997. Cell. 89 (6):981

GeneID: 16542
Latest Publications: View the latest CD309 articles on HighwirePress.com
UniProt: View information about CD309 on UniProt.org
Keywords: Purified anti-mouse CD309 (VEGFR2, Flk-1), Avas12, Purified, Flk-1, VEGF receptor-2, VEGF-R2, Ly-73, fetal liver kinase 1, Mouse, Immunology, Antibodies
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DescriptionCloneApplications
Purified Rat IgG2a, κ Isotype CtrlRTK2758FC, ICC, IF, IHC, IP, WB
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC

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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD309 (VEGFR2, Flk-1)
    Endothelial bEnd.3 cells stained with

    Endothelial bEnd.3 cells stained with Avas 12 PE

  • PE anti-mouse CD309 (VEGFR2, Flk-1)
    Endothelial bEnd.3 cells stained with

    Endothelial bEnd.3 cells stained with Avas 12 PE

  • APC anti-mouse CD309 (VEGFR2, Flk-1)
    bEnd.3 endothelial cells stained with

    bEnd.3 endothelial cells stained with Avas12a1 APC

  • Alexa Fluor® 488 anti-mouse CD309 (VEGFR2, Flk-1)
    bEnd.3 endothelial cells stained with

    bEnd.3 endothelial cells stained with Avas12a1 Alexa Fluor® 488

  • Biotin anti-mouse CD309 (VEGFR2, Flk-1)
    bEnd.3 mouse endothelial cells stained

    bEnd.3 mouse endothelial cells stained with biotinylated Avas12, followed by Sav-PE

  • PerCP/Cy5.5 anti-mouse CD309 (VEGFR2, Flk-1)
    bEnd.3 mouse endothelial cells stained

    bEnd.3 mouse endothelial cells stained with Avas12 PerCP/Cy5.5

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