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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 114108).
Application References:
1. Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC) 2. Personal Communication (WB) 3. Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim.) 4. Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block.) 5. Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim.) 6. Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
Con A-stimulated C57BL/6 splenocytes stained with purified RMP1-14, followed by biotinylated anti-rat IgG and Sav-PE
CD279 is a 50-55 kD immunoglobulin superfamily member, also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells).
Other Names:
Programmed Death-1, CD279
Structure:
Ig superfamily, 50-55 kD
Distribution:
Subset of double negative thymocytes, activated T and B cells
Function:
Lymphocyte clonal selection, peripheral tolerance
Ligand Receptor:
PD-L1 (B7-H1), PD-L2
Antigen References:
1. Barclay, A., et al. 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Agata, Y., et al. 1996. Int. Immunol. 8:765. 3. Nishimura, H., et al. 2001. Science 291:319. 4. Ishida, Y., et al. 1992. EMBO J. 11:3887.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD279 (PD-1)
Con A-stimulated C57BL/6 splenocytes stained with purified RMP1-14, followed by biotinylated anti-rat IgG and Sav-PE
LEAF™ Purified anti-mouse CD279 (PD-1)
Con A-stimulated C57BL/6 splenocytes stained with LEAF purified RMP1-14, followed by biotinylated anti-rat IgG and Sav-PE
