Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Workman C.J., et al., 2002. Eur. Immunol. 32:2255. 2. Workman, C.J. et al., 2005. 174:688. 3. Li, N., et al., 2004. J Immunol.173:6806.
Description:
CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.
Other Names:
LAG3, LAG-3
Structure:
Ig superfamily
Distribution:
T cells and NK cells, as well as T regulatory cells
Antigen References:
1. Baixeras, E. et al., 1992. J. Exp. Med. 176:327.
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