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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with purified CD206 (clone C068C2) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand Receptor:
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Antigen References:
1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469. 3. Burgdorf S, et al. 2006. J. Immunol. 176:6770. 4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD206 (MMR)
Thioglycollate-elicited Balb/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with biotinylated CD206 (clone C068C2) (top) or rat IgG2a, κ isotype control (bottom), followed by Sav-PE.
Purified anti-mouse CD206 (MMR)
Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with purified CD206 (clone C068C2) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
FITC anti-mouse CD206 (MMR)
Thioglycollate-elicited Balb/c macrophages were fixed/pemeabilized, and then stained with CD107b (Mac-3) APC and CD206 (clone C068C2) FITC (top) or rat IgG2a, κ FITC isotype control (bottom).
PE anti-mouse CD206 (MMR)
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) PE (top) or rat IgG2a, κ PE isotype control (bottom).
APC anti-mouse CD206 (MMR)
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).
Alexa Fluor® 488 anti-mouse CD206 (MMR)
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
Alexa Fluor® 647 anti-mouse CD206 (MMR)
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
